Multiplication of yellow fever virus in insect tissue cell cultures.

Yellow fever virus (Asibi strain) has been grown successfully in insect tissue culture cell lines from the moth Antheraea eucalypti and the mosquito Aedes aegypti. Increases in virus 1iter (MICLD5, of two to three logs were evident in 24 to 72 hours at 26 C in a hemolymph-free medium. Virusinfected A. eucalypti cells remained healthy and in a state of reproduction throughout 12 weeks' incubation, but in A. aegyyti cultures, cell lysis reduced the numbers to 10% of the original count within 120 hours.